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The spread of antibiotic resistance has become a serious threat to global public health. Recently, several studies have found that non-antibiotic chemicals can promote the generation and spread of bacterial antibiotic resistance. However, the effects of herbicides on the antibiotic resistance of bacteria remain unclear. In this study, Escherichia coli DH5α was used as the model strain to explore the effects of three commonly used herbicides (glyphosate, glufosinate, and dicamba) on the antibiotic resistance under soil environmental concen