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The transcription factor homeobox protein Hox‑B2 (HOXB2) and its downstream factor nucleolar and spindle‑associated protein 1 (NUSAP1) play important regulatory roles in cell proliferation, invasion and migration. However, their effects and specific mechanisms in nephroblastoma have not been previously investigated, to the best of our knowledge. Therefore, in the present study, the mRNA and protein expression levels of HOXB2 and NUSAP1 were determined in nephroblastoma cells using reverse transcription‑quantitative PCR and western blot a