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The development of a turn-on lanthanide luminescent probe for time-gated detection of nitroreductases (NTRs) in live bacteria is reported. The probe is activated through NTR-induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This novel NTR-responsive trigger is virtually non-fluorescent in its inactivated form and features a large signal increase upon activation. We show that the probe is capable of selectively sensing NTR in lysates as well as in live bacteria compr