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Zinc inhibited the cleavage of these host sequences in vitro. Other patterns emerged from multispecies sequence alignments of the cleavage sites, which may have implications for the selection of animal models and zoonosis. SSHHPS/nsP is an example of a sequence-specific post-translational silencing mechanism.Chemical modification of the prototype CB1R ago-PAM, GAT211, yielded new CB1R allosteric modulators (-)-(S,R)-13 and (+)-(R,S)-14, which showed significant bias for CB1R signaling pathways, as supported by docking studies. Compound 1