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PROSPERO) number CRD42020172251. International Prospective Register for Systematic Reviews (PROSPERO) number CRD42020172251. Professional support to enhance the early parent-infant relationship in the first months after birth is recommended, but little is known about the effect of universal interventions. The objective was to investigate the effect of health visitors' use of the Newborn Behavioral Observations system in new families. A cluster-randomised study was conducted in four Danish municipalities. Health visitors' geographical dis