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Glycosylation is a ubiquitous and important post-translational modification, functioning as a critical role in many biological processes. Among all the monosaccharides, sialic acids are of utmost importance, due to their relatively high abundance and association with various diseases. Elucidation of linkage isomers on sialic acids remains challenging. Various chemical derivatization techniques have been developed to identify the linkage isomers of sialic acids on released N-glycans, such as esterification and alkylamidation. Here, we

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