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This autofluorescence was detected in three of the four fluorescence channels restricting useful analysis to only one channel (red) on the instrument. The study highlights an important limitation to immunostaining techniques and reinforces the need for the use of a thorough set of controls to ensure specificity of quantitative analysis.New nitrogen and fluorine co-doped carbon dots were synthesized and used as a dual function fluorescent probe for silicon and mercury ions. The size of CDs was 10 nm. At optimum conditions (pH = 13, λex = 360
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