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1%). For MEC-EFS, enriched Ruminococcus and Geobacter in anodic biofilm might contribute to favorable biohydrogenation and electrochemical performance.Sargassum spp is an invasive macroalgae and an alternative feedstock for bioethanol production. Sargassum spp biomass was subjected to high-pressure technology for biomass fractionation under different operating conditions of temperature and residence time to obtain glucan enriched pretreated solids (32.22 g/100 g of raw material). Enzyme hydrolysis process at high pretreated solid loadin