https://www.selleckchem.com/pr....oducts/withaferin-a.
In this paper we have investigated and optimized a non-enzymatic fluorometric creatinine assay. The method was originally described by Blass in the 90s, but we found that besides the reagents mentioned in the paper, an addition of hydrogen peroxide is required to obtain a fluorescent compound. The excitation and emission maxima of the fluorophore are 405 nm and 475 nm, respectively. The optimal conditions for creatinine quantification are as follows 25 mmol L-1 3,5-dinitrobenzoic acid dissolved in 1,4-butanediol with 58 mmol L-1 H2
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