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54). In females, tooth loss and aging were significant explanatory variables for MMT (adjusted R2 = 0.36) and MMEI (adjusted R2 = 0.5), respectively. In both men and women, MMT and MMEI were highly correlated. CONCLUSIONS Masseter muscles in males were more likely to be attenuated by aging than in females. The main attenuation factors were observed to differ between MMT and MMEI in women. CLINICAL RELEVANCE In females, preservation of the natural dentition or prosthetic treatment may be effective for maintaining masseter muscle character