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pos target cells. Additionally, in total 663 T-cell clones (containing at least 91 unique clones expressing different T-cell receptors) directed against HLA*0201-restricted peptides of TAA WT1-RMF, RHAMM-ILS, Proteinase-3-VLQ, PRAME-VLD and NY-eso-1-SLL were isolated from HLA-A*0201pos donors. Only 3 PRAME-VLD- and 1 NY-eso-1-SLL-specific T-cell clone provoked IFN-gamma production and/or cytolysis upon stimulation with HLA-A*0201pos malignant cell lines (but not primary malignant samples) naturally overexpressing the TAA. These results
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