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The limits of detection, half-time and kinetics of the response are also presented. Higher temperatures and concentrations were found to increase the degree of the color change while decreasing the half-time of the response of the indicators. This work shows potential opportunities for the development of simple real-time amine indicators.With the wide application of cell metabolomics in many research areas, there is a need to develop an effective procedure for adherent mammalian cell metabolomics that allows for accurate determination o