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These neutralizing aspects limit the patient pools eligible for obtaining AAV-mediated therapies. AAV serotype 5 (AAV5) does not face the exact same buffer of humoral immunity as most AAV serotypes due to its reduced seroprevalence. However, AAV5 can only facilitate a decreased level of transgene appearance into the liver, constraining its application to only a few liver conditions. To boost the liver transduction of AAV5 while maintaining its low seroprevalence, we constructed a librar
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