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Leishmaniasis is an infectious disease common in tropical and subtropical regions caused by the genus , which is transmitted by the bite of female sandflies. In this study, we evaluate the anti-leishmanial effect of recombinant α-toxin protein alone and the combination with glucantime through in vitro and in vivo. Production, expression, and purification of recombinant α-toxin were evaluated by SDS-PAGE and Western blotting techniques. of the purified α-toxin plus and without glucantime were examined in vitro and in vivo. The results in

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