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Its application in cancer immunotherapy and precision oncology is blooming. Nevertheless, more effective methods of Cas protein delivery and the development of more accurate and efficient genome-editing tools are needed. The application of CRISPR-based therapeutic target screening is broadly used in cancer drug development. Its application in cancer immunotherapy and precision oncology is blooming. Nevertheless, more effective methods of Cas protein delivery and the development of more accurate and efficient genome-editing tools are need

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RG-I rich pectic polysaccharide is common in fruit and vegetable and possesses health benefits. However, it is removed during commercial pectin production because of poor gelling properties. Synergistic gelation can improve rheological properties of RG-I pectic polysaccharide and expand its application in functional food hydrocolloids. In the study, RG-I rich pectic polysaccharides at different degree of esterification was extracted from citrus membrane by sequential mild acidic (0.4% HCl, 28 °C) and alkaline (0.6% NaOH, 32 °C) treatment