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We discuss caveats and considerations in dFC analysis. Finally, we walk readers through an openly accessible toolbox to capture dFC properties and briefly review some of the dynamic metrics calculated using this toolbox.The ecological functions of core and non-core bacteria are gradually being identified, yet little is known about their responses to environmental changes and assembly processes, especially in urban river ecosystems. Here, we investigated bacterioplankton communities over 1 year in an urban section of the Ganjiang River, C