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The identified CC398 MRSA clones were PVL-positive ST1232-V (5C25), PVL-negative community-associated ST398-V (5C25) and livestock-associated ST398-V (5C25). Our findings demonstrate the importance of using WGS and comparing the sequences with international sequences to distinguish between CC398 CA-MRSA and LA-MRSA and to determine the isolates' origin. Furthermore, our findings suggest that CC398 CA-MRSA has become established in the Australian community and that ST398-V (5C25) LA-MRSA is now widespread in Australia