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fraction to be used for biofuel and biodiesel production in a single cultivation process, without the additional complexity of the nutrient starvation phase. Genome sequencing and identification of the mutations introduced in e8 mutant suggest possible genes responsible for the observed phenotypes, identifying putative target for future complementation and biotechnological application. © The Author(s) 2020.Background High-solids enzymatic hydrolysis has attracted increasing attentions for the production of bioethanol from lignocellulos