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A peak EQE of 21.6% is realized in the 2tCz2CzBn-based nondoped device with an extremely low turn-on voltage of 2.7 V, high color stability, a high brightness over 20 000 cd m-2, a narrow full-width at half-maximum of 70 nm, and CIE color coordinates of (0.167, 0.248). © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH Co. KGaA, Weinheim.Controlling the selective one-to-one conjugation of RNA with nanoparticles is vital for future applications of RNA nanotechnology. Here, the monofunctionalization of a gold nanoparticle (AuNP)