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An innovative new chromatography-based experimental way of identifying the designs of stereogenic centers β to nitrogen atoms in macrolactams ended up being set up and effectively applied in this report. These compounds exhibited significant bioactivities resistant to the silkworm entomopathogen Bacillus thuringiensis and various man pathogens along with man cancer mobile outlines. In particular, piceamycin potently inhibited Salmonella enterica and Proteus hauseri with MIC values of 0
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