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The level of anxiety and depression of patients with IBS-D was more serious than that in the control. The expression of NPS, NPY and NPY2R was down-regulated in the IBS-D. The total MC and tryptase-positive MC increased significantly in the colon tissue of IBS-D and the expression level of PAR-2 was significantly up-regulated. There has been a close connection among those indicators that the activated MC may up-regulate the function of PAR-2, resulting in thechange of neuropeptide (NPS and NPY), successively leading to clinical symptoms a
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