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Both PCR-based methods were highly reproducible and discriminative, with hexaplex-PCR fingerprinting being slightly more discriminative than rep-PCR typing. Overall, a high intra-species diversity was observed among the tested strains, indicating that further investigations on the strain level may be of interest.The emerging field of astropedology is the study of ancient soils on Earth and other planetary bodies. Examination of the complex factors that control the preservation of organic matter and other biosignatures in ancient soils is