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The antidiabetic effects and mechanisms of action of an analogue of a frog skin host-defence peptide belonging to the caerulein-precursor fragment family, [S4K]CPF-AM1 were investigated in db/db mice with a genetically inherited form of degenerative diabetes-obesity. Twice-daily treatment with the peptide (75 nmol/kg body weight) for 28 days significantly decreased blood glucose (P less then 0.01) and HbA1c (P less then 0.05) and increased plasma insulin (P less then 0.05) concentrations with no effect on body weight, energy intake, body c