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Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult. In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides. The analytical method was successfully developed using dextran standards up to 3755 kDa. This method was used to separate naturally occurring plant polysaccharides based on

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Additionally, only one computational technique that identifies whether piRNAs function in inducting target mRNA deadenylation already been reported into the literature. In this research, we created a two-layered built-in classifier algorithm, 2lpiRNApred. It identifies piRNAs in the first level and determines whether or not they function in inducting target mRNA deadenylation in the 2nd level. An innovative new feature choice algorithm, which