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Sequence analysis uncovered that Fm4CL2 is a type we 4CL gene associated with lignin biosynthesis. Analysis of mobile wall surface elements disclosed that Fm4CL2-overexpressing (OE-Fm4CL2) cigarette revealed increased lignin content (by 58.9%) and reduced hemicellulose content (by 41.2%). Detection of small-molecule metabolites into the lignin path revealed that coumaric acid content reduced by 48% and coniferyl liquor content increased by 250% compared with the control values. Weighed against wild ki