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Valvular calcification commonly occurs in elderly individuals, and is increasingly considered an important economic and health burden. However, no efficient drugs against valvular calcification are available. The present work aimed to examine emodin's suppressive effect on high-calcium-dependent valve calcification and explore the underpinning mechanisms. Experiments were carried out in mice receiving vitamin D (Vit D) to induce valvular calcification. Cell viability and apoptosis assays demonstrated celastrol suppressed proliferati