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In this report , we highlight the flexibility of a cell profiling method for actin turnover dynamics. Actin filaments in real time stem cells are labeled using SiR-actin, a cell permeable fluorogenic probe, to determine the endogenous actin turnover. Real time MSC imaging after times of induction effectively demonstrated lineage particular improvement in actin return. Next, we highlighted the distinctions when you look at the mobile heterogeneity of actin dynamics during adipogenic or ost