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Instrumental voice assessment plays a critical role in identifying vocal issues and for documenting treatment outcomes. The reported voice data, however, are sensitive to the algorithm used by each acoustic analysis software program (AASP) to analyze the corresponding waveform. In the present study, five acoustic measures were compared across healthy speakers and speakers with dysphonia for three AASPs commonly used in research, education, and clinical practice Multidimensional Voice Program (MDVP) by Computerized