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To develop a method for the determination of 13 antibiotics in 8 classes for desinfection products by ulta-high perfomance chromatography-tandem mass spectrometry(UPLC-MS/MS). Samples were extracted by methanol or acetonitrile. The target compouds were separated on a Waters HSS T3 column(100 mm×2. 1 mm, 1. 8 μm), and detected by triple quadrupole tandem mass spectrometer. The 13 selected antibiotics showed good linear relationships in the range of 4-100 μg/L and the correlation coefficients(r~2) were all above 0. 991. The limits of detect