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eters, potentially furthering CXR integration in patients' stratification. Metabolomics studies are not routine when quantifying amino acids (AA) in congenital heart disease (CHD). Comparative analysis of 24 AA in serum by traditional high-performance liquid chromatography (HPLC) based on ion exchange and ninhydrin derivatisation followed by photometry (PM) with ultra-high-performance liquid chromatography and phenylisothiocyanate derivatisation followed by tandem mass spectrometry (TMS); interpretation of findings in CHD patients and c