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Past research reports have primarily centered on managing double-stranded break dimensions, ssODN security, plus the DNA repair cycle. However, there is certainly too little study in the correlation amongst the cell period and single-strand template repair (SSTR) effectiveness. Right here, we investigated the relationship between mobile period and SSTR effectiveness. We found higher SSTR effectiveness during mitosis, particularly in the metaphase and anaphase. A Cas9 necessa