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97; 95% CI -7.05, -0.88). Cortisol concentration on a regular day was higher in males reporting incident anxiety (Beta 8.07; 95% CI 2.39, 13.76). The convenience sample with losses to follow-up may have led to a potential selection bias. Anxiety temporal patterns were associated with cortisol activity profiles in older adults with sex being a significant moderator. Future studies are recommended to ascertain the longitudinal changes in cortisol activity and anxiety temporal patterns, which may further inform personalized treatment of