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Results The new functions for assessing dendritic morphology were validated using primary mouse hippocampal and rat cortical neurons transfected with a fluorescently tagged MAP2 cDNA construct. These functions allow users to select specific populations of neurons as a training set for subsequent automated selection of labeled neurons in high-density cultures. Comparison with existing semi-automated methods Compared to manual or semi-automated analyses of dendritic arborization, the new functions increase throughput while significantly d