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Simulation making use of the calibrated design also obtained good fit for effluent COD focus, effluent nitrate concentration and bioreactor's MLSS focus of all three MBRs. The mean absolute percentage mistakes (MAPE) associated with the simulation mostly had been lower than 22%. The outcomes suggested that it is possible to utilizing BioWin, incorporated with appropriate dedication types of painful and sensitive variables, to simulate and monitor the procedure performance of MBR tre