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Roasted samples indicated more hardness due to change in morphology by heating. Clove bud with intact essential oil as such and its roasted form could be used as a natural antioxidant and antimicrobial agent in foodstuffs, particularly those containing lipid and can help to extend the shelf life of those food products, besides their health safety.D-amino acid oxidase (DAAO) is widely used in the industrial preparation of L-amino acids, and cultivating Escherichia coli (E. coli) expressing DAAO for the biosynthesis of L-phosphinothricin (L
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