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To evaluate the construct validity of Psychomotor Vigilance Test performance for measuring fatigue in people with acquired brain injury. Observational cross-sectional study. Fifty-four people with acquired brain injury and 61 healthy controls. Participants performed the Psychomotor Vigilance Test and reported momentary fatigue before and after this test and general fatigue. Associations between performance and fatigue in patients were tested by correlational and hierarchical multiple linear regression analyses, controlling for sleep qua