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Quetiapine was made use of as an interior standard (IS). Analytical split was done on ACQUITY UPLC C18 Column (2.1 × 100 mm; 1.7 μm) utilizing ammonium acetate (5 mM) in water and methanol ( with isocratic elution at ratio of (50, 50 v/v) and circulation rate of 0.4 mL/min. The length of time of split had been preserved for 3 min. Electrospray ionization mass spectrometry in a confident and unfavorable ionization mode was employed for detection. Discerning ion mode monitoring had been usef