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In this paper, four kinds of microfiber ball structure of different sizes, such as 330, 340, 350, and 360 µm were fabricated by single-mode fiber and multimode fiber, respectively. To test its performance, the reflected intensity was measured, and the results showed that the microfiber ball with the largest diameter of 360 µm had the maximum reflected intensity. The 360 µm microfiber ball was characterized by scanning electron microscope. This indicates that compared with a small-diameter microfiber ball, large-diameter microspheres are