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Surface roughness analysis was determined by Kruskal Wallis e Mann Whitney tests. RESULTS Regarding the mono-species biofilm, the cell viability and the metabolic activity showed that both chlorhexidine and red propolis had inhibitory effects and reduced the metabolism of biofilms, differing statistically from the growth control (p less then 0.05). With regards the co-culture biofilms, chlorhexidine had the highest inhibitory effect (p less then 0.05). The metabolic activity was reduced by the exposure to chlorhexidine and to red pr