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In addition, a dereplication study ended up being performed making use of UHPLC-HRMS and molecular networking, leading to the recognition of fifteen known compounds, being two sesquiterpene lactones and thirteen flavonoids. Some of the substances are now being described for the first time in L. brevifolius, as well as into the Lessingianthus genus.Improvements in the measurement and visual analysis of data, plotted across non-standardized graphs, tend to be feasible using the equations introduced