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Right here, we indicate that real properties of hydrogel material substrates determine P. aeruginosa biofilm structure. We show that hydrogel mesh size regulates twitching motility, a surface exploration method priming biofilms, finally controlling the organization of solitary cells in the multicellular community. The resulting architectural transitions increase P. aeruginosa's tolerance to colistin, a last-resort antibiotic drug. In addition, mechannces th
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