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Background Stool samples have been widely used to evaluate gut microbiota; however, little is known about the composition of human small intestinal microbiota and the alterations provoked by insulin resistance. Objective To describe the composition of jejunal microbiota in morbidly obese patients, as well as its link with insulin resistance and metformin treatment. Setting Virgen de la Victoria University Hospital and Regional University Hospital, Málaga, Spain. Methods Jejunal biopsies from 46 morbidly obese patients were analyzed by n