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We now have shown that silibinin treatment for 24 h in SCC-25 cells induced cytotoxicity in a concentration-dependent manner. The cytotoxic potential was as a result of the induction of apoptosis through the release of mitochondrial cytochrome c into the cytosol and subsequent activation of caspases-3 and -9. Twin staining assay had been further confirmed the induction of very early apoptosis upon silibinin treatment. Medicinal flowers have actually played a crucial

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