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The assay for the five analytes ended up being linear, stable, with imprecision significantly less than 9% and recoveries within ±10%. The low limits of measurement were 0.05, 0.05, 5, 0.025, and 0.025ng/mL for T, A4, DHEAS, DHT, and 17-OHP, correspondingly. Within the receiver running characteristic curve (ROC) analyses aided by the PCOS (n=63) and healthier (n=161) subjects, the AUC associated with the four-androgen combined had been greater than compared to any
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