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α-mangostin belongs to xanthone class of natural products, showing a great biological and pharmacological potential. α-mangostin has shown remarkable anticancer potential against different cancer cell lines. Herein, α-mangostin was tested for its anticancer potential against human ovarian cancer cell line (OVACAR-3). Its effects on reactive oxygen species (ROS), mitochondrial-mediated apoptosis, cell migration and invasion and m-TOR/PI3K/AKT signaling pathway, was also determined. MTT assay was performed to evaluate the rate of proliferati