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With the integrated AID, BG was controlled within the physiological range on average in 80% and 76% of the perfusion time for human and pig livers, respectively. The presented work offers a method and shows the feasibility to maintain BG in the physiological range for multiple (up to ten) days during ex situ liver perfusion with the help of an automated AID. Maintaining BG within the physiological range is required to enable long-term ex situ liver perfusion. Maintaining BG within the physiological range is required to enable long-term