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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor Francis Group on behalf of the Korean Society of Mycology.Two fungal species of ascomycetes were discovered during the screening of soil microflora from the Gangwon Province in Korea Didymella chlamydospora sp. nov. (YW23-14) and Microdochium salmonicolor sp. nov. (NC14-294). Morphologically, YW23-14 produces smaller chlamydospores (8.0-17.0 × 7.0-15.0 µm) than D. glomerata and D. musae. The strain NC14-294 was characterized by smaller conidiogenous cells (4.9-