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Three various kinds of surfactants had been tested anionic (SDBS and SDS), cationic (CTAB and MBC), and non-ionic (IGEPAL CA-210 and Tween 6. The total fatty acid content of Chlorella vulgaris ended up being 291.0 mg/g cell. Beneath the no-surfactant condition, the oil-extraction yield associated with the acid hydrothermal extraction ended up being 75.5%. The addition of SDBS and MBC at the 0.4% focus revealed improved oil-extraction overall performance, 85.4 and 85.7per cent yields, cor