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Due to the superior capabilities of microflow cytometry employing 3D focusing, this technology will be highly promising in the near future, with further applications in biology and medicine.Herein, we present a facile strategy for dopamine (DA) sensing by a water-stable MOF of [Tb(Cmdcp)(H2O)3]2(NO3)2·5H2On (1, H3CmdcpBr = N-carboxymethyl-(3,5-dicarboxyl)pyridinium bromide). Without any post-modification, MOF 1 functions as an effective fluorescent sensor for the label-free detection of DA with the detection limit of 0.41 μM (S/N = 3).