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Squid pen sample was treated by high hydrostatic pressure (HHP) prior to the extraction of chitosan. The physicochemical and antioxidant activities of the chitosan obtained with HHP (HHP-CS) were compared with chitosan of untreated squid pen sample (UT-CS). The chitosan extraction yield was optimized using response surface methodology, and the optimum condition was achieved at pressure of 500 MPa, extraction time of 10 min, and 1% (w/w) acetate concentration. The maximum yield of chitosan sample from the chitin of squid pens treated by
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